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rat anti gal3 mac2  (Cedarlane)


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    Structured Review

    Cedarlane rat anti gal3 mac2
    Rat Anti Gal3 Mac2, supplied by Cedarlane, used in various techniques. Bioz Stars score: 96/100, based on 398 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+anti+gal3+mac2/Anti-Mouse%2FHuman+Mac-2+(Galectin-3)%2C+Purified+(Clone+M3%2F38)+(rat+IgG2a)/pm41249504-231-89-93
    Average 96 stars, based on 398 article reviews
    rat anti gal3 mac2 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Microglial colonization of the developing mouse brain is controlled by both microglial and neural CSF-1
    Article Snippet: .. Slices were first incubated for 1 h at room temperature (RT) in 0.2% Triton X-100, 0.2% Gelatin in PBS (blocking solution), and then incubated in the same blocking solution with the following primary antibodies overnight at 4 °C: rat anti-CD206 (1/200; Bio-Rad Cat# MCA2235, RRID:AB_324622), chicken anti-GFP (1/1000; Aves Labs Cat# GFP-1020, RRID:AB_10000240), rabbit anti-IBA1 (1/500; FUJIFILM Wako Shibayagi Cat# 019-19741, RRID:AB_839504), chicken anti-IBA1 (1/400; Synaptic Systems Cat# 234 009, RRID:AB_2891282), rabbit anti-KI67 (1/200; Abcam Cat# ab15580, RRID:AB_443209), rat anti-Lyve1 (ALY7) (1/200; Thermo Fisher Scientific Cat# 14-0443-82, RRID:AB_1633414), rat anti-GAL3 (MAC2) (1/1000; Cedarlane Cat# CL8942AP; RRID:AB_10060357), goat anti-OPN (1/400; R and D Systems Cat# AF808, RRID:AB_2194992), goat anti-mouse Osteoactivin (GPNMB) (1/200; R and D Systems Cat# AF2330; RRID:AB_2112934), rabbit anti-P2RY12 (1/500; AnaSpec; EGT Group Cat# 55043 A, RRID:AB_2298886), and anti-VGlut2 (1/2000; Millipore Cat# AB2251-I, RRID:AB_2665454). .. Sections were rinsed in PBS 0.1% Triton X-100 and incubated from 2 to 18 h at 4 °C with secondary antibodies (1/400 in PBS, Jackson ImmunoResearch Labs): Alexa 10 Fluor® 488-conjugated donkey anti-chicken (Cat# 703-545-155, RRID:AB_2340375), Alexa 10 Fluor® 488-conjugated donkey anti-goat (Cat# 705-545-147, RRID:AB_2336933); Alexa 10 Fluor® 488-conjugated donkey anti-rat (Cat# 712-545-150, RRID:AB_2340683); Alexa 10 Fluor® 488-conjugated donkey rabbit (Cat# 711-545-152, RRID:AB_2313584); Cy3-conjugated donkey anti-goat (Cat# 705-165-147, RRID:AB_2307351), Cy3-conjugated donkey anti-rabbit (Cat# 711-165-152, RRID:AB_2307443), Cy3-conjugated donkey anti-rat (Cat# 712-165-150, RRID:AB_2340666), Alexa 10 Fluor® 647-conjugated donkey anti-goat (Cat# 705-605-147, RRID:AB_2340437), Cy5-conjugated donkey anti-goat (Cat# 705-175-147, RRID:AB_2340415), and Cy5-conjugated donkey anti-rat (Cat# 712-175-150, RRID:AB_2340671).

    Article Title: Microglial colonization of the developing mouse brain is controlled by both microglial and neural CSF-1.
    Article Snippet: .. Slices were first incubated for 1 h at room temperature (RT) in 0.2% Triton X-100, 0.2% Gelatin in PBS (blocking solution), and then incubated in the same blocking solution with the following primary antibodies overnight at 4 °C: rat anti-CD206 (1/200; Bio-Rad Cat# MCA2235, RRID:AB_324622), chicken anti-GFP (1/1000; Aves Labs Cat# GFP1020, RRID:AB_10000240), rabbit anti-IBA1 (1/500; FUJIFILM Wako Shibayagi Cat# 019-19741, RRID:AB_839504), chicken antiIBA1 (1/400; Synaptic Systems Cat# 234 009, RRID:AB_2891282), rabbit anti-KI67 (1/200; Abcam Cat# ab15580, RRID:AB_443209), rat anti-Lyve1 (ALY7) (1/200; Thermo Fisher Scientific Cat# 14- 0443-82, RRID:AB_1633414), rat anti-GAL3 (MAC2) (1/1000; Cedarlane Cat# CL8942AP; RRID:AB_10060357), goat anti-OPN (1/400; R and D Systems Cat# AF808, RRID:AB_2194992), goat anti-mouse Osteoactivin (GPNMB) (1/200; R and D Systems Cat# AF2330; RRID:AB_2112934), rabbit anti-P2RY12 (1/500; AnaSpec; EGT Group Cat# 55043 A, RRID:AB_2298886), and anti-VGlut2 (1/2000; Millipore Cat# AB2251-I, RRID:AB_2665454). .. Sections were rinsed in PBS 0.1% Triton X-100 and incubated from 2 to 18 h at 4 °C with secondary antibodies (1/400 in PBS, Jackson ImmunoResearch Labs): Alexa 10 Fluor 488-conjugated donkey anti-chicken (Cat# 703-545-155, RRID:AB_2340375), Alexa 10 Fluor 488-conjugated donkey anti-goat (Cat# 705-545-147, RRID:AB_2336933); Alexa 10 Fluor 488-conjugated donkey anti-rat (Cat# 712-545-150, RRID:AB_2340683); Alexa 10 Fluor 488-conjugated donkey rabbit (Cat# 711-545-152, RRID:AB_2313584); Cy3-conjugated donkey anti-goat (Cat# 705-165- 147, RRID:AB_2307351), Cy3-conjugated donkey anti-rabbit (Cat# 711-165-152, RRID:AB_2307443), Cy3-conjugated donkey anti-rat (Cat# 712-165-150, RRID:AB_2340666), Alexa 10 Fluor 647- conjugated donkey anti-goat (Cat# 705-605-147, RRID:AB_2340437), Cy5-conjugated donkey anti-goat (Cat# 705-175- 147, RRID:AB_2340415), and Cy5-conjugated donkey anti-rat (Cat# 712-175-150, RRID:AB_2340671).

    Blocking Assay:

    Article Title: Microglial colonization of the developing mouse brain is controlled by both microglial and neural CSF-1
    Article Snippet: .. Slices were first incubated for 1 h at room temperature (RT) in 0.2% Triton X-100, 0.2% Gelatin in PBS (blocking solution), and then incubated in the same blocking solution with the following primary antibodies overnight at 4 °C: rat anti-CD206 (1/200; Bio-Rad Cat# MCA2235, RRID:AB_324622), chicken anti-GFP (1/1000; Aves Labs Cat# GFP-1020, RRID:AB_10000240), rabbit anti-IBA1 (1/500; FUJIFILM Wako Shibayagi Cat# 019-19741, RRID:AB_839504), chicken anti-IBA1 (1/400; Synaptic Systems Cat# 234 009, RRID:AB_2891282), rabbit anti-KI67 (1/200; Abcam Cat# ab15580, RRID:AB_443209), rat anti-Lyve1 (ALY7) (1/200; Thermo Fisher Scientific Cat# 14-0443-82, RRID:AB_1633414), rat anti-GAL3 (MAC2) (1/1000; Cedarlane Cat# CL8942AP; RRID:AB_10060357), goat anti-OPN (1/400; R and D Systems Cat# AF808, RRID:AB_2194992), goat anti-mouse Osteoactivin (GPNMB) (1/200; R and D Systems Cat# AF2330; RRID:AB_2112934), rabbit anti-P2RY12 (1/500; AnaSpec; EGT Group Cat# 55043 A, RRID:AB_2298886), and anti-VGlut2 (1/2000; Millipore Cat# AB2251-I, RRID:AB_2665454). .. Sections were rinsed in PBS 0.1% Triton X-100 and incubated from 2 to 18 h at 4 °C with secondary antibodies (1/400 in PBS, Jackson ImmunoResearch Labs): Alexa 10 Fluor® 488-conjugated donkey anti-chicken (Cat# 703-545-155, RRID:AB_2340375), Alexa 10 Fluor® 488-conjugated donkey anti-goat (Cat# 705-545-147, RRID:AB_2336933); Alexa 10 Fluor® 488-conjugated donkey anti-rat (Cat# 712-545-150, RRID:AB_2340683); Alexa 10 Fluor® 488-conjugated donkey rabbit (Cat# 711-545-152, RRID:AB_2313584); Cy3-conjugated donkey anti-goat (Cat# 705-165-147, RRID:AB_2307351), Cy3-conjugated donkey anti-rabbit (Cat# 711-165-152, RRID:AB_2307443), Cy3-conjugated donkey anti-rat (Cat# 712-165-150, RRID:AB_2340666), Alexa 10 Fluor® 647-conjugated donkey anti-goat (Cat# 705-605-147, RRID:AB_2340437), Cy5-conjugated donkey anti-goat (Cat# 705-175-147, RRID:AB_2340415), and Cy5-conjugated donkey anti-rat (Cat# 712-175-150, RRID:AB_2340671).

    Article Title: Microglial colonization of the developing mouse brain is controlled by both microglial and neural CSF-1.
    Article Snippet: .. Slices were first incubated for 1 h at room temperature (RT) in 0.2% Triton X-100, 0.2% Gelatin in PBS (blocking solution), and then incubated in the same blocking solution with the following primary antibodies overnight at 4 °C: rat anti-CD206 (1/200; Bio-Rad Cat# MCA2235, RRID:AB_324622), chicken anti-GFP (1/1000; Aves Labs Cat# GFP1020, RRID:AB_10000240), rabbit anti-IBA1 (1/500; FUJIFILM Wako Shibayagi Cat# 019-19741, RRID:AB_839504), chicken antiIBA1 (1/400; Synaptic Systems Cat# 234 009, RRID:AB_2891282), rabbit anti-KI67 (1/200; Abcam Cat# ab15580, RRID:AB_443209), rat anti-Lyve1 (ALY7) (1/200; Thermo Fisher Scientific Cat# 14- 0443-82, RRID:AB_1633414), rat anti-GAL3 (MAC2) (1/1000; Cedarlane Cat# CL8942AP; RRID:AB_10060357), goat anti-OPN (1/400; R and D Systems Cat# AF808, RRID:AB_2194992), goat anti-mouse Osteoactivin (GPNMB) (1/200; R and D Systems Cat# AF2330; RRID:AB_2112934), rabbit anti-P2RY12 (1/500; AnaSpec; EGT Group Cat# 55043 A, RRID:AB_2298886), and anti-VGlut2 (1/2000; Millipore Cat# AB2251-I, RRID:AB_2665454). .. Sections were rinsed in PBS 0.1% Triton X-100 and incubated from 2 to 18 h at 4 °C with secondary antibodies (1/400 in PBS, Jackson ImmunoResearch Labs): Alexa 10 Fluor 488-conjugated donkey anti-chicken (Cat# 703-545-155, RRID:AB_2340375), Alexa 10 Fluor 488-conjugated donkey anti-goat (Cat# 705-545-147, RRID:AB_2336933); Alexa 10 Fluor 488-conjugated donkey anti-rat (Cat# 712-545-150, RRID:AB_2340683); Alexa 10 Fluor 488-conjugated donkey rabbit (Cat# 711-545-152, RRID:AB_2313584); Cy3-conjugated donkey anti-goat (Cat# 705-165- 147, RRID:AB_2307351), Cy3-conjugated donkey anti-rabbit (Cat# 711-165-152, RRID:AB_2307443), Cy3-conjugated donkey anti-rat (Cat# 712-165-150, RRID:AB_2340666), Alexa 10 Fluor 647- conjugated donkey anti-goat (Cat# 705-605-147, RRID:AB_2340437), Cy5-conjugated donkey anti-goat (Cat# 705-175- 147, RRID:AB_2340415), and Cy5-conjugated donkey anti-rat (Cat# 712-175-150, RRID:AB_2340671).



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